I have found the biggest changes in tone will come from v1 in the pre & your power tubes.
Basically v1 is your noticable distortion. Your power tubes are your drive & how loud your amp can go cleanly/ headroom. You will notice the differences in power tube tone as you increase the volume. All get warmer with volume. Some have extended treble response, some have extended treble response without distorting. Some will distort earlier giving more drive & others will just keep getting louder without much distortion @ all.
Changing tubes will not alter the fundamental tone of the amp. They can help with small annoying tonal issues though.
Amps like the 5150 rely on a balance of the pre & power section to generate tone. This however can lead to issues as when you find the perfect tone, you can't move anything & everyone else has to work around you.
Amps like the pittbull & Diezel tend to generate most of their tone through the pre section, meaning you can wind the volume knob around & the tone stays cosistant other than it gets a little warmer with more volume.
becuase these amps don't rely on the power tubes as much, they tend to look after them a lot better.
This is less of a problem with el34 based amps as they are designed to sound great loud as that is where the el34 tube is designed to operate. Loud & hot.
The Diezel Herbert fixes all these issues by, having a kick ass pre section on all channels, running 6 power tubes so they don't get overworked. It can run el34, 6L6 & kt88. But it's fucking awseome feature is that it can run 2 el34's, 2 6L6's & 2 kt88's @ THE SAME TIME. Giving you the best of all worlds.